Detection of fumonisin B1 by aptamer-functionalized magnetic beads and ultra-performance liquid chromatography

• Published in: Microchemical journal 2022-07, Vol.178, p.107346, Article 107346
• Main Authors: Deng, Mei, Li, Weiqiang, Chen, Yanbiao, Wang, Keyu, Sun, Yifan, Xu, Hengyi
• Format: Article
• Language: English
• Subjects: Aptamer; Fumonisin B1; Magnetic beads; PEG; UPLC
• ISSN: 0026-265X; 1095-9149
• DOI: 10.1016/j.microc.2022.107346

[Display omitted] •Apt-PEG-MBs for separation of FB1 is a alternative to immunoaffinity columns.•Apt-PEG-MBs-UPLC provides a specific and cost-efficient assay for FB1 detection.•The mediation system based on PEG can increase the load of Apt and reduce cost. Mycotoxin contamination endangers human health and life through the whole food supply chain. The monitoring of mycotoxin pollution requires reliable and sensitive detection methods. Conventional Ultra Performance Liquid Chromatography (UPLC) with immunoaffinity column suffers from the limitations of low sensitivity and efficiency. To overcome these limitations, we developed a strategy based on polyethylene glycol (PEG) mediated and aptamer (Apt) functionalized magnetic beads (MBs) to enrich and separate fumonisin B1 (FB1), which was subsequently detected through UPLC. The established strategy showed a good linear relationship when the concentration range of FB1 was 1–70 ng/mL. The feasibility of this strategy had been verified with the spiked corn flour samples, and achieved high specificity and excellent accuracy with a limit of detection (LOD) as low as 3.75 ng/mL. In addition, PEG was introduced into the system as a mediator, which could reduce steric hindrance and the cost. And the established platform has the potential to be applied for determination of other mycotoxins by changing Apt.