Introducing uracil-containing oligo to improve exponential amplification reaction performance in biosensor application

[Display omitted] •The uracil-containing oligo changed the enzyme kinetics of DNA polymerase.•The uracil-containing oligo reduced non-specific amplification.•This method improved the detection limit of EXPAR.•This method enhanced the distinguishability of EXPAR. Exponential amplification reaction (E...

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Bibliographic Details
Published in:Microchemical journal 2023-08, Vol.191, p.108866, Article 108866
Main Authors: Deng, Yuhan, Zhang, Zhen, Zhou, Yujie, Pei, Xiaojing, He, Yifan, Wu, Tongbo
Format: Article
Language:English
Subjects:
Background reduction
Exponential amplification reaction (EXPAR)
Highly sensitive detection
Polymerase
Uracil-containing oilgos
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Summary:[Display omitted] •The uracil-containing oligo changed the enzyme kinetics of DNA polymerase.•The uracil-containing oligo reduced non-specific amplification.•This method improved the detection limit of EXPAR.•This method enhanced the distinguishability of EXPAR. Exponential amplification reaction (EXPAR) has the advantages of high detection sensitivity and short detection time, but it also has the problem of high background signal. In this paper, we innovatively added the uracil-containing oligo that could inhibit the non-specific activity of Vent (exo-) DNA polymerase to increase the detection effect of EXPAR.
ISSN:0026-265X
1095-9149
DOI:10.1016/j.microc.2023.108866