A novel ratiometric electrochemical immunosensor for the detection of cancer antigen 125 based on three-dimensional carbon nanomaterial and MOFs

• Published in: Microchemical journal 2024-05, Vol.200, p.110372, Article 110372
• Main Authors: Wu, Fangfang, Gao, Hongmin, Qiu, Ren, Zhang, Hehua, Ren, Xinshui, Qi, Xue, Miao, Men, Rui, Chuang, Chang, Dong, Pan, Hongzhi
• Format: Article
• Language: English
• Subjects: Cancer antigen 125; Ratiometric electrochemical immunosensor; Three-dimensional reduced graphene oxide/multi-walled carbon nanotube; UiO-66-NH2
• ISSN: 0026-265X; 1095-9149
• DOI: 10.1016/j.microc.2024.110372

A novel ratiometric electrochemical immunosensor for the detection of cancer antigen 125 based on three-dimensional carbon nanomaterial and MOFs. [Display omitted] •Three-dimensional carbon nanomaterial and MOFs were constructed as a ratiometric electrochemical immunosensor for CA125 detection.•The framework structures of 3DrGO/MWCNTs and UIO-66-NH2 can amplify signals with a substantial loading of molecules and enhancement of electrochemical active surface area.•The ratiometric dual-signal and signal amplification strategies improve sensor stability and sensitivity for a reference for future ratiometric electrochemical sensors. Accurate and sensitive detection of cancer antigen 125 (CA125) is important in early ovarian cancer diagnosis and monitoring. In the present work, a novel electrochemical ratiometric immunosensor for the determination of CA125 based on three-dimensional reduced graphene oxide/multi-walled carbon nanotube carboxylic acid- thionine (3DrGO/MWCNTs-Thi) and UIO-66-NH2/ferrocenecarboxylic acid (UiO-66-Fc) was constructed. 3DrGO/MWCNTs-Thi of immobilized primary antibody (Ab1) and UiO-66-Fc of labeled second antibody (Ab2) were used as capture probes and signaling probes for CA125, respectively. As the concentration of CA125 increases, the oxidation peak currents (IThi and IFc) generated by the two materials were changed in opposite directions, and therefore the ratio of the currents (IThi/IFc) was negatively correlated with the concentration of CA125. Thanks to the amplification of high loading of signaling molecules by the framework nanocomposites and the ratiometric strategy, the constructed electrochemical immunosensor exhibits outstanding accuracy, stability, and sensitivity. Under optimal conditions, the detection range spanned from 0.01 U mL−1 to 80 U mL−1, with a calculated limit of detection (LOD) of 0.0089 U mL−1. The immunosensor was successfully applied to real serum sample tests, indicating its potential for clinical detection applications.