An unlabeled electrochemical sensor for the simultaneous detection of homocysteine and uric acid based on molecularly imprinted recognition for prediction of cardiovascular disease risk

• Published in: Microchemical journal 2024-05, Vol.200, p.110376, Article 110376
• Main Authors: Liu, Miao, Tang, Shanshan, Wang, Yuwei, Liang, Axin, Luo, Aiqin
• Format: Article
• Language: English
• Subjects: Cardiovascular disease; Electrochemical sensor; Homocysteine; Molecularly imprinted polymer; Uric acid
• ISSN: 0026-265X
• DOI: 10.1016/j.microc.2024.110376

[Display omitted] •An electrochemical sensor was prepared for simultaneous detection of Hcy and UA.•MIP based on electropolymerization ensured the specific detection of Hcy and UA.•The sensor showed a wider detection range and lower detection limit for Hcy and UA.•The sensor was successfully applied to detect Hcy and UA in serum samples. An unlabeled molecular imprinted electrochemical sensor was developed for simultaneous detection of homocysteine and uric acid, based on gold nanoparticles and molecularly imprinted polymer composites modified glass carbon electrode. Gold nanoparticles stably enhanced the electron transfer and improved the sensitivity by acting as a base for enhancing signal for molecularly imprinted polymers. Specific molecular imprinting cavities based on electropolymerization with dopamine were formed to specifically and simultaneously identify homocysteine and uric acid. Combining the good sensitivity of electrochemistry and the excellent selectivity of molecularly imprinted polymer, the difference in peak current position was used to achieve simultaneous detection of homocysteine and uric acid. For this reason, the developed sensor showed the wide detection range of 5.00 × 10−3-1.00 × 103 μmol/L for homocysteine and 5.00 × 10−1-5.00 × 103 μmol/L for uric acid, with the LOD of 5.64 × 10−5 μmol/L and 0.128 μmol/L (S/N = 3). The developed sensor with easy preparation, great selectivity and short detection time could be used in simultaneous detection of homocysteine and uric acid in serum.