Ternary deep eutectic Solvents-Based Three-Phase partitioning for speciation of Cr (Ⅲ) and Cr (Ⅵ) in edible liquid Oil: A promising method for substituting

• Published in: Microchemical journal 2024-09, Vol.204, p.110965, Article 110965
• Main Authors: Liu, Bangfu, Zhou, Le-zhou, Deng, Xiaobin, Tian, Xiangrong, He, Guo-wen, Wang, Chaoli, yang, Fan
• Format: Article
• Language: English
• Subjects: Deep eutectic solvents; Edible liquid oil; Liquid–liquid microextraction; Speciation analysis of Chromium; Three-phase partitioning
• ISSN: 0026-265X; 1095-9149
• DOI: 10.1016/j.microc.2024.110965

[Display omitted] •In this study, the main highlights is showed as following:•Ternary DESs was succeeded prepared by choline chloride, ethylene glycol and glycerol. And DES-4 was selected as optimum. And antimicrobial experiment proved that the DES-4 is green.•Cr (III) and Cr (VI) were succeeded extracted by DES-4. The one-offs extraction yield is 95.0%, 2 times is about 100%.•Cr (III) and Cr (VI) were succeeded complete separation using H3PO4/KH2PO4 saturated buffer solution to remain pH = 4.5 in the chemical reactions.•The developed method was succeeded to analyse Cr (III) and Cr (VI) in peanut oil and with satisfactory results. In this paper, a novelty ternary deep eutectic solvents was prepared with choline chloride, ethylene glycol and glycerol. The pH value was adjust to develop three-phase for speciation of Cr (Ⅲ) and Cr (Ⅵ) extract and separate in ganoderma lucidum spore oil based on ternary deep eutectic solvents. The results show that DES-4 has a good extraction of Cr (III) and Cr (VI), the sample-extraction ratio was 3:1, Cr (III) was protected by EDTA, all chromium ions in ganoderma lucidum spore oil was extracted by near 100 % in 2 times, Cr (III) and Cr (VI) was well separated by saturated buffer solution of KH2PO4/H3PO4 (pH = 4.5). The LOD is 0.031 mg/kg. The RSD is 4.1 %. The recovery between 85.2 % and 111.5 %. This method was successfully applied to detect speciation chromium in other edible liquid oil.