HPLC-UV method for the quantification of a novel indole-thiazole derivative (CS08) in nanoparticles

• Published in: Microchemical journal 2024-09, Vol.204, p.111047, Article 111047
• Main Authors: Soares, José Cleberson Santos, Cavalcanti, Iago Dillion Lima, Cruz-Filho, Iranildo José da, Lira Nogueira, Mariane Cajubá de Britto, Lima, Maria do Carmo Alves de
• Format: Article
• Language: English
• Subjects: Drug quantification; Indole-thiazole derivatives; Nanoparticles; Polysaccharides; Validation
• ISSN: 0026-265X; 1095-9149
• DOI: 10.1016/j.microc.2024.111047

[Display omitted] •The method for quantifying the new indole-thiazole derivative by HPLC-UV was selective, linear, accurate and robust.•The method was effective in assessing the stability of CS08 at different pHs.•It was also possible to use the method to identify the encapsulation efficiency of CS08 in polymeric nanoparticles. Validation of an analytical method for the quantification of a drug candidate molecule is the first step. The quantification method is fundamental in the pharmaceutical industry, as it allows the development of a pharmaceutical product, such as encapsulation in nanocarriers. To validate the quantification method of a novel indole-thiazole derivative (CS08) by HPLC-UV in nanoparticles. We developed a quantification method using an HPLC coupled to a UV detector, and evaluated the method for its selectivity, linearity, precision, accuracy, LOD, LOQ, and robustness. With the method, we evaluated the stability of CS08 in different pHs simulating biological media and quantified the encapsulation rate in dextran- or fucoidan-coated nanoparticles. It was possible to quantify CS08 using the method in different matrices, proving to be a selective, linear, precise, accurate, and robust method in the determined concentrations (1–20 µg.mL−1). The LOD and LOQ were low on a microgram scale, which facilitates the quantification of CS08 for the development of pharmaceutical formulations, showing confidence in the results obtained from the rate of encapsulation in nanoparticles. The validated method proved to be adequate for the quantification of CS08 in different matrices, allowing the evaluation of its encapsulation rate in nanoparticles.