Quantitative determination of Lactobacillus acidophilus in combination with Azithromycin, Cefixime, Ofloxacin

• Published in: Microchemical journal 2024-10, Vol.205, p.111117, Article 111117
• Main Authors: Sathala, PavanKumar, Lalchandani, Dimple S., Chenkual, Laltanpuii, Sonone, Prajyot R., Upmanyu, Neeraj, Porwal, Pawan Kumar
• Format: Article
• Language: English
• Subjects: Azithromycin; Cefixime; FT-IR; Lactobacillus acidophilus; Ofloxacin; Partial least square regression (PLSR) model; Probiotics
• ISSN: 0026-265X
• DOI: 10.1016/j.microc.2024.111117

[Display omitted] •The sensitive determination of Lactobacillus Acidophilus in presence of cefixime, ofloxacin and azithromycin.•Two PLS models were developed and optimized for quantitative determination.•Traditional HPLC-PDA method was developed for simultaneous determination of cefixime and ofloxacin.•Noble HPLC-ELSD method was developed for determination of Azithromycin.•FTIR-PLS METHOD show pose less or almost no-challenges over traditional HPLC for determination of Lactobacillus Acidophilus. Fourier-transform infrared spectroscopy (FTIR) combined with multicomponent multivariate quantitative models plays a crucial role and provides a successful alternative in analyzing achromic, complex, and molecular structures with diverse properties. In the present research work, we had discussed the quantitative determination of Azithromycin, Cefixime, Ofloxacin, and Lactobacillus acidophilus using a simple, economical, and accurate FT-IR spectroscopy method combined with partial least square (PLS) analysis. The full spectrum FTIR-based PLS models were developed for randomly distributed concentration sets and cross-validated for untested concentration determination. For each analyte, the concentrations were chosen over a linearity range of 1–5 % w/w, which encompasses the concentration ratios in the market samples. The root mean PRESS value and % error for validation concentration sets was observed at 0.12, for the PLS model for full spectrum scan-randomly distributed concentration sets. Chromatographic methods for the determination of AZT, Cefixime, Ofloxacin, and Lactobacillus acidophilus was also developed. The chromatographic retention of AZT was achieved on Phenomenex Luna 5 µm C18 column with solvents A: 25 % (20 mM Ammonium Acetate (pH-5.7)) & B: 75 % (Acetonitrile) as mobile phase in isocratic mode and was developed and validated using HPLC-ELSD detection. Whereas quantitative determination of Cefixime and Ofloxacin was determined simultaneously on YMC-Triart C18 column and the gradient consisted of solvent-A (20 mM Disodium hydrogen phosphate (pH-5.5), solvent-B (Acetonitrile) & solvent-C (Methanol) using HPLC-DAD detection. The FTIR-PLS method yielded significant benefits over traditional HPLC methods, including superior accuracy and precision, ease of experimentation, and quantitative determination of “difficult to analyse” probiotics in combination with antibiotics.