Development of ultra-sensitive and selective molecularly imprinted polymer-based electrochemical sensor for L-lactate detection

• Published in: Microchemical journal 2024-09, Vol.204, p.111163, Article 111163
• Main Authors: Piskin, Ensar, Cetinkaya, Ahmet, Eryaman, Zülal, Karadurmus, Leyla, Unal, Mehmet Altay, Sezgintürk, Mustafa Kemal, Hizal, Julide, Ozkan, Sibel A.
• Format: Article
• Language: English
• Subjects: L-lactate; Molecularly imprinted polymers; Nanoparticle; Photopolymerization; Voltammetry
• ISSN: 0026-265X; 1095-9149
• DOI: 10.1016/j.microc.2024.111163

[Display omitted] •Design of a new nanomaterial-supported MIP-based sensor for L-LAC determination.•Application with high sensitivity to detect L-LAC in commercial serum samples.•Modification of the electrode surface with ZIF-8@ZnQ to raise surface area.•The excellent repeatability, reproducibility, very low LOD values, and selectivity. Lactate detection is important for the food and healthcare industries, and it’s especially important when there’s tissue hypoxia, hepatic illness, bleeding, respiratory failure, or sepsis. A new molecularly imprinted polymer (MIP) based electrochemical sensor was fabricated for differential pulse voltammetric assay of L-lactate (LAC). By using ZIF-8@ZnQ nanoparticles, the number of regions and effective surface area were increased. The polymeric film was obtained using 4 aminobenzoic acid (4-ABA) as a functional monomer, ethylene glycol dimethacrylate (EGDMA) as a cross-linker, 2-hydroxyethyl methacrylate (HEMA) as basic monomers, and 2-hydroxy-2-methylpropiophenone one as initiator. The developed 4-ABA/LAC/ZIF-8@ZnQ@MIP-GCE was morphologically characterised using SEM and electrochemically using electrochemical impedance spectroscopy (EIS) and cyclic voltammetry (CV) measurements. A linear range of 0.1–1.0 pM LAC with a detection limit of 29.9 fM was found. Lastly, the MIP-based electrochemical sensor detected LAC in commercial human serum samples.