Rapid screening of Salmonella typhimurium based on a bacterial surface-imprinted polymer-functionalized bipolar electrode-electrochemiluminescence platform

• Published in: Microchemical journal 2024-12, Vol.207, p.111788, Article 111788
• Main Authors: Li, Mengjuan, Qi, Yan, Xu, Mingyao, Wang, Fengyang, Xiong, Xiaohui, Liu, Yuanjian
• Format: Article
• Language: English
• Subjects: BPE-ECL; S. typhimurium; SIP
• ISSN: 0026-265X
• DOI: 10.1016/j.microc.2024.111788

A bipolar electrode-electrochemiluminescence (BPE-ECL) sensor, utilizing the distinctive properties of a polydopamine (PDA) bacterial surface-imprinted polymer (SIP), has been skillfully developed for the highly sensitive detection of Salmonella typhimurium (S. typhimurium). [Display omitted] •A BPE-ECL platform based on bacterial surface imprinted polymer was constructed for S. typhimurium detection.•Fc-aptamer-S. typhimurium as a signal probe specifically recognized S. typhimurium.•A low detection limit of 10 CFU mL−1S. typhimurium could be identified. A bipolar electrode-electrochemiluminescence (BPE-ECL) sensor, utilizing a polydopamine (PDA) bacterial surface-imprinted polymer (SIP), has been developed for the detection of Salmonella typhimurium (S. typhimurium). To fabricate this sensor, dopamine (DA) served as the monomer, while S. typhimurium played a crucial role as the template molecule. Through a precision-tuned electropolymerization process, a polymer film template was seamlessly crafted on the cathode of BPE, encasing the target bacteria within its matrix. Once the template was delicately removed, a molecular surface-imprinted film emerged on the cathode, forming a PDA SIP capture probe that was remarkably adept at recognizing and binding to the target bacteria. When the target bacteria was present on the cathode, the Fc-aptamer-S. typhimurium acted as a signal probe to specifically recognized S. typhimurium. Applying a constant potential to the BPE, the oxidation product Fc+, generated at the cathode, accelerating the luminescence of the [Ru(bpy)3]2+/TPrA system on the anode. The sensor exhibited a remarkable sensitivity, capable of detecting S. typhimurium concentrations ranging from 101 to 106 CFU mL−1, with a detection limit of 10 CFU mL−1.