β-Cyclodextrin functionalized persistent luminescence nanoparticle for autofluorescence-free detection of α-glucosidase activity and its inhibitor

• Published in: Microchemical journal 2024-12, Vol.207, p.112211, Article 112211
• Main Authors: Zhang, Hong-Jiao, He, Li-Wei, Dong, Ke-Min, Li, Song-Yuan, Luo, Can, Han, Jia-Qi, Li, Rui
• Format: Article
• Language: English
• Subjects: Autofluorescence-free analysis; Inhibitor acarbose; Persistent luminescence; α-glucosidase; β-cyclodextrin
• ISSN: 0026-265X
• DOI: 10.1016/j.microc.2024.112211

A autofluorescence-free sensing strategy based on β-cyclodextrin functionalized persistent luminescence nanoparticle is developed for detection of α-glucosidase activity and its inhibitor with high selectivity and sensitivity. [Display omitted] •A persistent luminescence nanoprobe PLNP-β-CD is prepared.•Nanoprobe leads to sensing without autofluorescence interference.•The detection system provides high selectivity and sensitivity.•The method permits α-glucosidase and its inhibitor detection. The development of simple, selective and sensitive analytical methods to effectively monitor α-glucosidase (α-Glu) and discover its new inhibitors is very critical for the diagnosis and treatment of diabetes. Herein, an autofluorescence-free sensing method based on β-cyclodextrin (β-CD) functionalized persistent luminescence nanoparticle (PLNP) is established for the detection of α-Glu activity and its inhibitor. The p-nitrophenol (PNP) produced by the hydrolysis of substrate p-nitrobenzene-α-D-glucopyranoside (PNPG) by α-Glu in the system can be selectively captured by the β-CD on the nanoprobe to form adducts through the host guest effect between them, resulting in persistent luminescence quenching due to electron transfer (ET) effect. The proposed method provides a good linear relationship within the range of 5–80 U/L and a low detection limit of 0.9 U/L, and can detect α-Glu in human serum with the recovery rate of 95.4–103.3 %. Furthermore, the detection system can measure the inhibitory ability of acarbose with an IC50 value of 349 μM, proving its good potential for application.