Competitive adsorption of lysozyme and myoglobin on mesostructured cellular foam silica

A model of multicomponent multilayer adsorption has been developed to describe the simultaneous adsorption of lysozyme (14.3 kDa, pI = 11.35) and myoglobin (17.6 kDa, pI = 7.2) on mesostructured cellular foam (MCF) silica in 0.02 M potassium phosphate buffer at pH 7 and 25 °C. The mechanism of adsor...

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Bibliographic Details
Published in:Microporous and mesoporous materials 2015-07, Vol.210, p.101-109
Main Authors: Darwish, Amina, Robie, Taylor, Desch, Rebecca J., Thiel, Stephen W.
Format: Article
Language:English
Subjects:
Adsorption model
Guggenheim–Anderson–deBoer (GAB)
Lysozyme
Multicomponent
Myoglobin
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Summary:A model of multicomponent multilayer adsorption has been developed to describe the simultaneous adsorption of lysozyme (14.3 kDa, pI = 11.35) and myoglobin (17.6 kDa, pI = 7.2) on mesostructured cellular foam (MCF) silica in 0.02 M potassium phosphate buffer at pH 7 and 25 °C. The mechanism of adsorption was proposed based on batch adsorption isotherms and flow microcalorimetry (FMC) traces for the enthalpy of adsorption. Thermodynamic derivations of Guggenheim–Anderson–deBoer (GAB) and Langmuir models were used to develop the model based on the proposed mechanism. Regression analysis demonstrated the effect of each of the proposed mechanistic equations. [Display omitted] •A model of multicomponent multilayer adsorption was developed.•The model includes competitive, cooperative, and multilayer adsorptions.•The model is consistent with adsorption data and measured enthalpies of adsorption.•A single parameter was needed to account for lysozyme–myoglobin interactions.
ISSN:1387-1811
1873-3093
DOI:10.1016/j.micromeso.2015.02.010