Staphylococcus epidermidis biofilm quantification: Effect of different solvents and dyes

Staphylococcus epidermidis biofilm formed in the presence of the solvents DMSO, ethanol or methanol was quantified using safranin or crystal violet staining protocols. We found that biofilm quantification was the most accurate when safranin protocol was applied. Moreover, both DMSO and ethanol stimu...

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Bibliographic Details
Published in:Journal of microbiological methods 2014-06, Vol.101, p.63-66
Main Authors: Wu, X., Santos, R.R., Fink-Gremmels, J.
Format: Article
Language:English
Subjects:
Biofilms - drug effects
Coloring Agents - pharmacology
Crystal violet
Dimethyl Sulfoxide
DMSO
Ethanol
Gentian Violet
Methanol
Phenazines
Safranin
Solvents - pharmacology
Staphylococcus epidermidis - drug effects
Staphylococcus epidermidis - isolation & purification
Staphylococcus epidermidis - physiology
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Summary:Staphylococcus epidermidis biofilm formed in the presence of the solvents DMSO, ethanol or methanol was quantified using safranin or crystal violet staining protocols. We found that biofilm quantification was the most accurate when safranin protocol was applied. Moreover, both DMSO and ethanol stimulated biofilm formation. •We compare DMSO, ethanol and methanol on bacterial biofilm formation.•We evaluate the sensitivity of safranin and crystal violet as biofilm dyes.•We evaluate decolorization of dyes by Staphylococcus epidermidis.•Methanol does not affect biofilm formation and safranin was the best dye.•DMSO and ethanol stimulates biofilm and crystal violet dye may mislead results.
ISSN:0167-7012
1872-8359
DOI:10.1016/j.mimet.2014.03.016