Molecular characterization of Salmonella Typhimurium isolated in Brazil by CRISPR-MVLST

• Published in: Journal of microbiological methods 2017-02, Vol.133, p.55-61
• Main Authors: Almeida, Fernanda, Medeiros, Marta Inês Cazentini, Rodrigues, Dália dos Prazeres, Allard, Marc W., Falcão, Juliana Pfrimer
• Format: Article
• Language: English
• Subjects: Alleles; Bacterial Typing Techniques; Brazil; Clustered Regularly Interspaced Short Palindromic Repeats - genetics; CRISPR-MVLST; DNA, Bacterial - isolation & purification; Food Contamination - analysis; Food Microbiology; Genetic Loci; Humans; Multilocus Sequence Typing; PFGE; Salmonella Typhimurium; Salmonella typhimurium - genetics; Salmonella typhimurium - isolation & purification; Sequence Analysis, DNA
• ISSN: 0167-7012; 1872-8359
• DOI: 10.1016/j.mimet.2016.12.020

CRISPR-multi-locus virulence sequence typing (CRISPR-MVLST) was performed to type 92 S. Typhimurium strains isolated from humans and food sources between 1983 and 2013 in Brazil and assess the suitability of this methodology comparing it with PFGE already used for subtyping the same strains. Among the 92 S. Typhimurium strains studied, we identified 25 CRISPR1 alleles, 27 CRISPR2 alleles, 2 fimH alleles and 3 sseL alleles showing that the genetic variability is much higher in the CRISPRs loci than in the virulence genes. The CRISPR-MVLST analysis provided similar results to the PFGE previously published used to type the same set of strains, demonstrating that CRISPR-MVLST is a very efficient approach for subtyping S. Typhimurium serovar and can be used to complement and validate results obtained by the PFGE methodology. •Subtyping of S. Typhimurium isolated over 30years in Brazil by CRISPR-MVLST.•CRISPR-MVLST provided additional information than did only the CRISPR analysis.•CRISPR-MVLST provided similar results to the ones obtained by PFGE.•CRISPR-MVLST can be used to complement and validate results obtained by PFGE.