Application of ddPCR for detection of Enterococcus spp. in coastal water quality monitoring

Droplet digital polymerase chain reaction (ddPCR) was evaluated for the detection of fecal indicator bacteria (FIB), Enterococcus spp., in San Diego County beach water samples collected under diverse conditions, from multiple pollution sources, as part of regulatory monitoring activities over 20 mon...

Full description

Saved in:
Bibliographic Details
Published in:Journal of microbiological methods 2021-05, Vol.184, p.106206, Article 106206
Main Authors: Crain, Chad, Kezer, Keith, Steele, Syreeta, Owiti, Judith, Rao, Sphoorthy, Victorio, Maria, Austin, Brett, Volner, Alon, Draper, William, Griffith, John, Steele, Joshua, Seifert, Marva
Format: Article
Language:English
Subjects:
AB411
Bacteria - genetics
Bacteria - growth & development
Bacteria - isolation & purification
Droplet digital polymerase chain reaction
Enterococci
Environmental Monitoring - methods
Feces - microbiology
Humans
Intrinsic copy number equation
Quantitative polymerase chain reaction
Real-Time Polymerase Chain Reaction - methods
Seawater - chemistry
Seawater - microbiology
United State Environmental Protection Agency
United States
United States Environmental Protection Agency
Water Pollution - analysis
Water Quality
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Droplet digital polymerase chain reaction (ddPCR) was evaluated for the detection of fecal indicator bacteria (FIB), Enterococcus spp., in San Diego County beach water samples collected under diverse conditions, from multiple pollution sources, as part of regulatory monitoring activities over 20 months. Two US EPA-approved methods, qPCR (EPA 1609.1) and Enterolert (SM9230D), were used as reference comparator methods. A total of 361 samples were assayed by both ddPCR and qPCR and yielded an acceptable Index of Agreement (IA) of 0.89, based on EPA Site-Specific analysis guidelines. A Pearson's correlation coefficient of r = 0.87 (p 
ISSN:0167-7012
1872-8359
DOI:10.1016/j.mimet.2021.106206