Expression differential of microsomal and cytosolic glutathione-S-transferases in Fasciola hepatica resistant at triclabendazole

[Display omitted] ► The cytosolic GST activity has increased in the TCBZ resistant strain. ► The microsomal GST activity has increased in the TCBZ resistant strain. ► This increased activity was 53% for the cytosolic fraction and 29% for microsomal fraction. ► Fluke resistant overexpressed predomina...

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Bibliographic Details
Published in:Molecular and biochemical parasitology 2012-01, Vol.181 (1), p.37-39
Main Authors: Scarcella, S., Lamenza, P., Virkel, G., Solana, H.
Format: Article
Language:English
Subjects:
adults
Animals
Anthelmintics - metabolism
Benzimidazoles - metabolism
biochemical pathways
Cytosol - enzymology
Drug Resistance
Fasciola hepatica
Fasciola hepatica - drug effects
Fasciola hepatica - enzymology
flukes
gene expression regulation
Glutathione Transferase - biosynthesis
Glutathione-S-transferases
Metabolic activity
Microsomes - enzymology
parasitology
Triclabendazole
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Summary:[Display omitted] ► The cytosolic GST activity has increased in the TCBZ resistant strain. ► The microsomal GST activity has increased in the TCBZ resistant strain. ► This increased activity was 53% for the cytosolic fraction and 29% for microsomal fraction. ► Fluke resistant overexpressed predominantly the cytosolic fraction. ► Fluke resistant is a response multienzymatic involving several metabolic pathway. In the present work, we evaluate in vitro the cytosolic and microsomal activity of glutathione-S-transferases in adults of Fasciola hepatica susceptible (Cullompton Strain) and resistant (Sligo Strain) to triclabendazole. The triclabendazole resistant (Sligo) fluke expressed significant major metabolic activity of glutathione-S-transferases compared to that measured in the cytosolic and microsomal fractions obtained from susceptible flukes (Cullompton Strain). The results associated with previous data where the Sligo Strain overexpress Flavin Monooxigenase confirm a multienzymatic response involving more than one metabolic pathway.
ISSN:0166-6851
1872-9428
DOI:10.1016/j.molbiopara.2011.09.011