Octadecanoic acid/silica particles synthesis for enzyme immobilization: Characterization and evaluation of biocatalytic activity

Water insoluble enzyme particles (WIEP) were prepared by first anchoring the octadecanoic acid over porous silica particles (OAS) followed by diastase enzyme binding. These particles were characterized using FT-IR, X-ray crystallography and X-ray photoelectron spectroscopy (XPS) and evaluated for st...

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Bibliographic Details
Published in:Journal of molecular catalysis. B, Enzymatic Enzymatic, 2008-09, Vol.55 (1), p.43-48
Main Authors: Prakasham, R.S., Likhar, Pravin R., Rajyalaxmi, K., Subba Rao, Ch, Sreedhar, B.
Format: Article
Language:English
Subjects:
Bioconversions. Hemisynthesis
Biological and medical sciences
Biotechnology
Enzyme
Enzyme activity
Fundamental and applied biological sciences. Psychology
Immobilization
Methods. Procedures. Technologies
Silica
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Summary:Water insoluble enzyme particles (WIEP) were prepared by first anchoring the octadecanoic acid over porous silica particles (OAS) followed by diastase enzyme binding. These particles were characterized using FT-IR, X-ray crystallography and X-ray photoelectron spectroscopy (XPS) and evaluated for starch hydrolysis activity. The results indicated that the enzyme binding to OAS particles was dependent on pH and temperature of the medium. Maximum enzyme binding (>48%) to OAS particles was observed at pH 3 and at 30 °C, while highest biocatalytic activity of WIEP was observed at pH 4 and at 40 °C. The storage stability study of water insoluble enzyme particles was evaluated for more than 70 days without any enzyme activity loss.
ISSN:1381-1177
1873-3158
DOI:10.1016/j.molcatb.2008.01.008