Enzymatic synthesis of palm-based ascorbyl esters

The synthesis of palm-based ascorbyl esters through transesterification of ascorbic acid and palm oil in tert-amyl alcohol catalyzed by immobilized lipase is described. Highest conversion (70–75%) was determined after 16 h reaction at 40 °C using lipase (Novozyme 435 from Candida antartica) with an...

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Bibliographic Details
Published in:Journal of molecular catalysis. B, Enzymatic Enzymatic, 2009-06, Vol.58 (1), p.153-157
Main Authors: Burham, Hamidah, Rasheed, Raizatul Ainaa Gafoor Abdul, Noor, Noorullhamezon Md, Badruddin, Suzaini, Sidek, Hamidah
Format: Article
Language:English
Subjects:
Antioxidant
Bioconversions. Hemisynthesis
Biological and medical sciences
Biotechnology
Fundamental and applied biological sciences. Psychology
Methods. Procedures. Technologies
Novozyme 435
Palm oil
Palm-based ascorbyl esters
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Summary:The synthesis of palm-based ascorbyl esters through transesterification of ascorbic acid and palm oil in tert-amyl alcohol catalyzed by immobilized lipase is described. Highest conversion (70–75%) was determined after 16 h reaction at 40 °C using lipase (Novozyme 435 from Candida antartica) with an ascorbic acid to palm oil mole ratio of 1:8. The purified product was further characterized by 13C NMR and GC–MS and the mixture of ascorbyl monoesters obtained were identified as ascorbyl monooleate (61%), ascorbyl monopalmitate (30%) and ascorbyl monostearate (9%). The antioxidant activity of palm-based ascorbyl esters was evaluated by 1,1-diphenyl-2-picrylhydrazyl (DPPH) test. The results showed that pure palm-based ascorbyl esters have an antioxidant activity with an IC 50 value of 0.1 mg/mL.
ISSN:1381-1177
1873-3158
DOI:10.1016/j.molcatb.2008.12.012