Immobilization and stabilization of microbial lipases by multipoint covalent attachment on aldehyde-resin affinity: Application of the biocatalysts in biodiesel synthesis

• Published in: Journal of molecular catalysis. B, Enzymatic Enzymatic, 2011, Vol.68 (1), p.109-115
• Main Authors: Mendes, Adriano A., Giordano, Roberto C., Giordano, Raquel de L.C., de Castro, Heizir F.
• Format: Article
• Language: English
• Subjects: Bioconversions. Hemisynthesis; Biodiesel; Biological and medical sciences; Biotechnology; Fundamental and applied biological sciences. Psychology; Lipases; Methods. Procedures. Technologies; Multipoint covalent attachment; Resin affinity; Thermal-stability
• ISSN: 1381-1177; 1873-3158
• DOI: 10.1016/j.molcatb.2010.10.002

▪. ▶ Thermomyces lanuginosus lipase (TLL) immobilized by multipoint covalent attachment on glyoxyl-resin allowed obtaining derivatives with the highest hydrolytic activity (olive oil emulsion hydrolysis) and thermal stability. ▶ Lipase from Pseudomonas fluorescens (PFL) showed to be more active in ester synthesis than olive oil emulsion hydrolysis. ▶ Immobilized lipases on resin activated with glutaraldehyde undergo strong distortion of the enzyme three-dimensional structure. ▶ Highest conversion into biodiesel was found in the transesterification of palm oil catalyzed by both TLL and PFL glyoxyl-derivatives. Microbial lipase preparations from Thermomyces lanuginosus (TLL) and Pseudomonas fluorescens (PFL) were immobilized by multipoint covalent attachment on Toyopearl AF-amino-650M resin and the most active and thermal stable derivatives used to catalyze the transesterification reaction of babassu and palm oils with ethanol in solvent-free media. For this, different activating agents, mainly glutaraldehyde, glycidol and epichlorohydrin were used and immobilization parameters were estimated based on the hydrolysis of olive oil emulsion and butyl butyrate synthesis. TLL immobilized on glyoxyl-resin allowed obtaining derivatives with the highest hydrolytic activity (HA der) and thermal stability, between 27 and 31 times more stable than the soluble lipase. Although PFL derivatives were found to be less active and thermally stables, similar formation of butyl butyrate concentrations were found for both TLL and PFL derivatives. The highest conversion into biodiesel was found in the transesterification of palm oil catalyzed by both TLL and PFL glyoxyl-derivatives.