Enhancing the performance of a phospholipase A1 for oil degumming by bio-imprinting and immobilization

• Published in: Journal of molecular catalysis. B, Enzymatic Enzymatic, 2016-01, Vol.123, p.122-131
• Main Authors: Li, Zhemin, Liu, Hui, Zhao, Genhai, Wang, Peng, Wang, Li, Wu, Hefang, Fang, Xue, Sun, Xiaowen, Wu, Xihua, Zheng, Zhiming
• Format: Article
• Language: English
• Subjects: Bio-imprinting; Enzymatic degumming; Immobilization; Miscella; Phospholipase
• ISSN: 1381-1177; 1873-3158
• DOI: 10.1016/j.molcatb.2015.11.018

[Display omitted] •Activity and substrate specificity of phospholipase A1 in hexane media were enhanced by bio-imprinting.•Activity of imprinted-phospholipase A1 in hexane media was further improved by immobilization on diatomite.•Crude soybean oil could be highly efficiently degummed by immobilized-imprinted-phospholipase A1.•Immobilized-imprinted-phospholipase A1 could be used in oil/hexane miscella degumming before desolventizing. When phospholipase A1 (PLA1) was bio-imprinted with 5% soybean lecithin at pH 5.0, its phospholipase (PLA) activity in lecithin–hexane solution increased by 30.9-fold and its substrate specificity was substantially enhanced. A further increase in PLA activity of the bio-imprinted PLA1 (bi-PLA1) from 562U/g to 1288U/g was obtained when 105mg diatomite/mg bi-PLA1 was incorporated as an immobilization carrier. The degumming abilities of PLA1, bi-PLA1, and immobilized bi-PLA1 (im-bi-PLA1) in crude soybean oil and crude oil–hexane (1:1 w/w) miscella were also investigated. The phosphorus content in the crude soybean oil was reduced to 7.3mg/kg from an initial level of 406.9mg/kg after incubation with 100mg/kg im-bi-PLA1 and 2% water at 50°C for 180min. In miscella degumming, the residual phosphorus content was reduced from 406.9mg/kg to 18.7mg/kg by incubation with 100mg/kg im-bi-PLA1 and 2% water at 50°C for 180min and neutralization with 3M NaOH solution. In comparison, the original PLA1 reduced the residual phosphorus content to 62.9mg/kg and 92.4mg/kg in crude oil and miscella degumming, respectively.