Surfactants and fatty alcohol based novel nanovesicles for resveratrol: Process optimization, characterization and evaluation of functional properties in RAW 264.7 macrophage cells

• Published in: Journal of molecular liquids 2018-07, Vol.261, p.387-396
• Main Authors: Vankayala, Jaya Sravani, Battula, Surendra Nath, Kandasamy, Ruckmani, Mariya, Gover Antoniraj, Franklin, Magdaline Eljeeva Emerald, Pushpadass, Heartwin Amaladhas, Naik, Laxmana Naik
• Format: Article
• Language: English
• Subjects: Fatty alcohol; Intracellular ROS scavenging activity; MTT assay; Nanovesicles; Resveratrol
• ISSN: 0167-7322; 1873-3166
• DOI: 10.1016/j.molliq.2018.04.058

Resveratrol, a natural polyphenol widely applauded for its cardio-protective and anti-cancer activity, has limited application due to its poor solubility and low bioavailability. Hence, an attempt was made to optimize the preparation of biocompatible resveratrol loaded niosomes (R-niosomes) using non-ionic surfactants and fatty alcohol as stabilizer employing the novel and green method of sonication. R-Niosomes exhibited mean particle size of 108nm with narrow size distribution (PDI=0.24) and high zeta potential (−56.8mV). The niosomes had >80% of resveratrol encapsulated into the vesicles and exhibited controlled release under simulated gastro-intestinal conditions. FTIR, XRD and DSC analyses confirmed successful encapsulation of resveratrol into the vesicles. Assays in macrophage RAW264.7 cell lines showed that R-niosomes had lower cytotoxicity and higher intracellular ROS scavenging activity when compared to resveratrol in its free form. This study demonstrated that nanoencapsulation of resveratrol through niosomes using non-ionic surfactant and fatty alcohol has dramatically improved its solubility and functionality. [Display omitted] •Resveratrol-niosomes were prepared by sonication using cetyl alcohol as stabilizer.•Nanovesicles were characterized by SEM, TEM, FTIR, DSC and XRD techniques.•Nanoencapsulated resveratrol exhibited less cytotoxicity than the free resveratrol.•Enhanced ROS scavenging activity observed with the encapsulated form of resveratrol.•Resveratrol nanovesicles had better functional activity than its free form.