Characterization of an anionic membrane mimetic with natural phospholipid content and magnetic orienting capabilities

The cellular membrane is a highly complex and dynamical structure, with variable and inhomogeneous composition, which makes detailed structural studies a very difficult task. 2H-NMR has become one of the most useful tools for many of such studies, however this requires the use of simpler structures...

Full description

Saved in:
Bibliographic Details
Published in:Journal of molecular liquids 2021-02, Vol.323, p.114616, Article 114616
Main Authors: Muñoz-Gacitúa, D., Monroy-Cárdenas, Matias, Araya-Maturana, R., Weiss-López, B.
Format: Article
Language:English
Subjects:
2H-RMN
Membrane mimetic
Molecular dynamics
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:The cellular membrane is a highly complex and dynamical structure, with variable and inhomogeneous composition, which makes detailed structural studies a very difficult task. 2H-NMR has become one of the most useful tools for many of such studies, however this requires the use of simpler structures that behave as close as possible to the cellular membrane, i.e. membrane mimetics. In this article we present a new anionic nematic lyotropic liquid crystal, with an important content of a natural mixture of phospholipids isolated from soybean, which can be employed as membrane mimetic. The capability of the mesophase to spontaneously orient when exposed to an external magnetic field, enables it for studies about distribution, dynamics and mobility of mesophase components, as well as dissolved substrates, employing 2H-NMR. A 2H-NMR characterization of the mimetic is performed by using different deuterated probes. To validate its permeability properties as membrane, the permeating capability of Benzocaine and the inability of Levodopa to do so were tested. For an atomic detailed characterization of both, the new mimetic and the process of crossing the interface, we also present a molecular dynamics simulation calibrated to reproduce experimental 2H-NMR results. [Display omitted] •High content of natural phospholipid: 52.5% of the total mass of the mimetic.•Benzocaine can translocate the membrane mimetic, as it would on a natural membrane.•Levodopa is unable to translocate passively the membrane mimetic, as expected.•Molecular dynamics model calibrated to reproduce 2H NMR results.
ISSN:0167-7322
1873-3166
DOI:10.1016/j.molliq.2020.114616