Influence of natural deep eutectic systems in water thermal behavior and their applications in cryopreservation

Natural deep eutectic systems (NADES), which have applications as solvents for both engineering and life sciences, are mainly composed of sugars, aminoacids or organic acids. In this work NADES composed by glucose, urea and proline (G:U:P in a molar ratio of 1:1:1) and proline and glucose (P:G 5:3)...

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Bibliographic Details
Published in:Journal of molecular liquids 2021-05, Vol.329, p.115533, Article 115533
Main Authors: Craveiro, R., Castro, V.I.B., Viciosa, M.T., Dionísio, M., Reis, R.L., Duarte, Ana Rita C., Paiva, A.
Format: Article
Language:English
Subjects:
Cytotoxicity
Glucose
NADES
Proline
Thermal properties
Urea
Water mixtures
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Summary:Natural deep eutectic systems (NADES), which have applications as solvents for both engineering and life sciences, are mainly composed of sugars, aminoacids or organic acids. In this work NADES composed by glucose, urea and proline (G:U:P in a molar ratio of 1:1:1) and proline and glucose (P:G 5:3) were prepared and added in different mass fractions to water. By differential scanning calorimetry it was verified as the crystallization tendency of water is modified even for low fraction of NADES added. This is also observed by polarized optical microscopy which allowed following the formation of crystals with different crystalline morphologies as bulk water. Calorimetric data also shown as the crystallization temperature decreases for all P:G mixtures and this shift is more accentuated for weight fraction of NADES higher than 0.5. Crystallization is totally suppressed for NADES fraction higher than 0.7. NADES/water mixtures cytotoxicity was evaluated in vitro, revealing that they are less toxic as compared with the commonly used cryoprotective additives as dimethyl sulfoxide (DMSO). Additionally, cell viability tests with cell lines cryopreserved using DMSO and both the prepared NADES showed comparable viability. This work combines thermophysical data on NADES and evaluates it’s in vitro performance, providing cues for their use in cryopreservation applications. •NADES composed of glucose (G), urea (U) and proline (P) were prepared.•G:U:P in a molar ratio (1:1:1) and P:G (5:3).•NADES influence in water thermal behavior was studied by DSC and POM.•NADES induce expressive decrease in melting and crystallization temperature of water.•Cytotoxicity was assessed, being NADES less toxic than cryoprotectant DMSO.
ISSN:0167-7322
DOI:10.1016/j.molliq.2021.115533