Optimization and application of green solvent extraction of natural bioactive coumarins from Lamiaceae and Asteraceae herbal plants

• Published in: Journal of molecular liquids 2021-09, Vol.338, p.116691, Article 116691
• Main Authors: Hroboňová, Katarína, Jablonský, Michal, Májek, Pavel
• Format: Article
• Language: English
• Subjects: Extraction; Herbal plants; Low transition temperature mixture; Optimization; Simple coumarins
• ISSN: 0167-7322; 1873-3166
• DOI: 10.1016/j.molliq.2021.116691

•The effect of LTTMs composition on coumarins extraction was verified.•The recovery reached higher than 87% under the optimized conditions.•Efficient method with ecological LTTM extraction and HPLC determination of coumarins was developed. Simple coumarins, 2H-1-benzopyran-2-ones, represent group of natural bioactive compounds. They are considered as secondary metabolites in plants of Lamiaceae and Asteraceae families. In this study, heat assisted extraction with low transition temperature mixtures (LTTM) based on choline chloride and L-lactic acid were used for simultaneous extraction of esculin, umbelliferone, scoparone, 4-methylumbelliferone and herniarin from herbal plants prior their determination by HPLC method with fluorescence detection. The effect of LTTM composition on coumarins extraction was verified. Optimal extraction conditions included choline chloride/L-lactic acid/1,3-propanediol (1:2:1) extraction solvent, solid/liquid ratio 1/40 g/mL, treatment time 15 min, rotation speed 20 rpm and temperature 60 °C. Temperature was the most significant parameter affecting extraction efficiency, the extraction time was an important variable for penetration of solvent in the matrix. Proposed HPLC method was applicable in linear concentration ranges from 5 to 100 ng/mL (for esculin, umbelliferone, scoparone, 4-methylumbelliferone) and from 15 ng/mL to 100 ng/mL (for herniarin) (R2 ≥ 0.998). The limits of detection were 0.2–5.0 ng/mL. The recovery of the method was higher than 87.7% (RSD