Development of novel fluorescent probes to detect and quantify specific reactive oxygen species

• Published in: Journal of molecular liquids 2023-01, Vol.369, p.120884, Article 120884
• Main Authors: Szymaszek, Patryk, Środa, Patrycja, Tyszka-Czochara, Małgorzata, Chachaj-Brekiesz, Anna, Świergosz, Tomasz, Ortyl, Joanna
• Format: Article
• Language: English
• Subjects: Cytotoxicity; Fluorescence; Probe; Reactive oxygen species (ROS)
• ISSN: 0167-7322; 1873-3166
• DOI: 10.1016/j.molliq.2022.120884

[Display omitted] •Novel fluorescent probe based on pyridine-coumarin were synthetized successfully.•Probe displays fluorescence turn-on response in the presence of H2O2.•It offer good performances in terms of sensitivity and response time.•Cytotoxicity studies on various model cell lines proved the non-toxic activity of the tested sensor. Hydrogen peroxide (H2O2) plays important roles in redox signaling and oxidative stress, and its dynamic concentration is critical to human health and diseases. Here we report the synthesis of a novel fluorescent probe based on pyridine-coumarin core 3-(2-pyridyl)-7-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)chromen-2-one (P182) for quantitative measurement of H2O2. This fluorometric probe displays a fluorescence turn-on response in the process of deprotection of arylboronate to phenol in the presence of H2O2. It could offer good performances in terms of sensitivity and response time. Moreover, cytotoxicity studies on various model cell lines proved the non-toxic activity of the tested sensor allowing its use in vivo studies. This study provides research on molecular fluorescence probe for the detection of H2O2.