The identification of microorganisms by micro-Raman spectroscopy

The identification of microorganisms in low amounts (monolayers, single cells) has been achieved by means of Raman spectroscopy. In a first example, we investigate single bacteria colonies on Agar plates by means of micro-Raman spectroscopy. Secondly, we demonstrate the spatially resolved distinctio...

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Bibliographic Details
Published in:Journal of molecular structure 2003-12, Vol.661, p.363-369
Main Authors: Rösch, P., Schmitt, M., Kiefer, W., Popp, J.
Format: Article
Language:English
Subjects:
Bacteria
Micro-Raman spectroscopy
Raman mapping
Yeast
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Summary:The identification of microorganisms in low amounts (monolayers, single cells) has been achieved by means of Raman spectroscopy. In a first example, we investigate single bacteria colonies on Agar plates by means of micro-Raman spectroscopy. Secondly, we demonstrate the spatially resolved distinction between a smear of two colored microorganism on a glass plate. Finally, we show the possibility to detect extremely small amounts of colorless bacteria with the confocal micro Raman technique.
ISSN:0022-2860
1872-8014
DOI:10.1016/j.molstruc.2003.06.004