Synthesis, characterization, DNA-Binding, enzyme inhibition and antioxidant studies of new N-methylated derivatives of pyridinium amine

• Published in: Journal of molecular structure 2017-06, Vol.1137, p.84-96
• Main Authors: Zafar, Muhammad Naveed, Perveen, Fouzia, Nazar, Muhammad Faizan, Mughal, Ehsan Ullah, Rafique, Humera, Tahir, Muhammad Nawaz, Akbar, Muhammad Sharif, Zahra, Sabeen
• Format: Article
• Language: English
• Subjects: Antioxidant; DNA binding; Enzyme inhibition; N-methylated; Pyridinium amine
• ISSN: 0022-2860; 1872-8014
• DOI: 10.1016/j.molstruc.2017.02.035

A series of novel N-methylated derivatives of pyridinium amine, [L1][Tf]-[L5][Tf], were synthesized and characterized by FTIR, NMR, MS and XRD analyses. Preliminary biological screening of these compounds including antioxidant, enzyme inhibition and DNA (salmon sperm) interaction studies were also carried out. The targeted compounds were synthesized by a melt reaction between 4-chloro-N-methyl pyridinium triflate and corresponding amines (1-naphthyl amine, o-ansidine, 2-nitroaniline, p-ansidine and cyclohexyl amine) at temperature of 230 °C. The DPPH radical antioxidant scavenging activities of these compounds at maximum concentration of 50 μg/mL were observed in the range of 60–70%. Acetylcholine esterase (AChE) and Butylcholine esterase (BChE) inhibitory activities of synthesized compounds at 2 mM concentration were also measured to be at maximum of 79 and 71% respectively. The spectral behavior of ligand-DNA obtained from photo-luminescent measurements showed that all ligands bind with DNA via non-covalent interactions. The binding constant values were determined by UV–visible and fluorescence spectroscopy and were quite close to that obtained from molecular docking studies. •Synthesis and Characterization of five new N-methylated pyridinium amine compounds.•DNA Interaction studies of these newly synthesized compounds with UV-visible and florescence studies.•Close observed binding constants (Kb) and binding energies (ΔGb°) calculated with both techniques.•Antioxidant and enzyme inhibition activities showed remarkable results in each case.•In-depth Analysis by Molecular docking and QSAR studies.