Fluorescence and dynamics studies of dye-biomolecule interaction in the nano-colloidal systems

• Published in: Journal of molecular structure 2019-01, Vol.1175, p.821-827
• Main Authors: Rahdar, Abbas, Najafi-Ashtiani, Hamed, Sanchooli, Esmael
• Format: Article
• Language: English
• Subjects: Deaggregation; Dye; Dynamic light scattering; Fluorescence; Interaction; Water nano-droplet
• ISSN: 0022-2860; 1872-8014
• DOI: 10.1016/j.molstruc.2018.08.046

The design and development the fluorescent sensors with effective sensitivity is a key challenge in the sensing studies. In the current work, deaggregation of the Rhodamine B (RhB) and Acridine Orange (AO) dyes within the bulk water and water nano-droplet was studied by adding biomolecules of bovine serum albumin and trypsin to the mentioned systems by using fluorescence, light scattering, and FT-IR techniques. Fluorescence studies focus on dye-biomolecule interactions indicated a deaggregation in fluorescence behavior of dye from non-fluorescent to fluorescent form after adding biomolecules of BSA and trypsin to the bulk aqueous solution of RhB and water-in-oil AOT (Aerosol-OT or Bis (2-ethylhexyl) sulfosuccinate sodium salt) micro-emulsion (ME) containing RhB at a constant dye concentration, separately. The dynamic light scattering (DLS) study of AOT nano-droplets ME containing RhB indicated that the nano-droplet size decreased as the fluorescence emission intensity of RhB increased. [Display omitted] •The deaggregation of dye was studied in the bulk water and nano-micelles.•The fluorescence of dye increased after addition of BSA to solution.•The size of nano-micelles decreased after addition of BSA to micelle.