Functionalized polyaniline based on protonic acid doping as a direct electron mediator to amplify sensor signals

A “label-free” electrochemical immunosensor was developed to detect α-fetoprotein (AFP) sensitively. HCS@PANI (High carbonized nanospheres@Polyaniline) is used as an electrochemical sensing platform to improve conductivity and enhanche the amount of AFP antibody (anti-AFP) immobilized on the electro...

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Bibliographic Details
Published in:Journal of molecular structure 2020-06, Vol.1209, p.127924, Article 127924
Main Authors: Zhao, Chulei, Gao, Ke, Ma, Chaoyun, Wu, Mei, Cao, Kaihang, Yang, Yuxiao, Hong, Chenglin, Qiao, Xiuwen
Format: Article
Language:English
Subjects:
AFP
HCS
PANI
Protonic acid
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Summary:A “label-free” electrochemical immunosensor was developed to detect α-fetoprotein (AFP) sensitively. HCS@PANI (High carbonized nanospheres@Polyaniline) is used as an electrochemical sensing platform to improve conductivity and enhanche the amount of AFP antibody (anti-AFP) immobilized on the electrode surface. PANI, which is involved by protonic acid, exhibits excellent electrochemical activity and can be used as a direct electron mediator to further amplify electrochemical signals. Under optimal conditions, the electrochemical immunosensor has a linear range of 0.001–80 ng/mL and a minimum detection limit of 0.33 pg/mL. The immunosensor is expected to be used in clinical laboratories to detect AFP and other tumor markers in human plasma for precancerous screening or cancer surveillance. •Polyaniline modified by protonic acid exhibits excellent electrochemical activity, utilizing its reactive group covalent bond and antibody.•HCS removes organic compounds from the surface during calcination to ensure its biocompatibility and form uniform nanospheres.
ISSN:0022-2860
1872-8014
DOI:10.1016/j.molstruc.2020.127924