Highly selective detection of citrulline using curcumin analogue and its application in food samples with logic gate behaviour

• Published in: Journal of molecular structure 2023-09, Vol.1288, p.135771, Article 135771
• Main Authors: Lavanya, Raja, Srinivasadesikan, Venkatesan, Lin, Ming-chang, Padmini, Vediappen
• Format: Article
• Language: English
• Subjects: Biomolecules; Citrulline; Curcumin; EDTA and DFT calculation; Lower detection limit
• ISSN: 0022-2860; 1872-8014
• DOI: 10.1016/j.molstruc.2023.135771

•A simple fluorescent compound (5HPD) was synthesized for the detection of Citrulline (Cit).•The probe(5HPD) shows highly sensitivity and selectivity towards Citrulline.•1H NMR titration, FTIR, MS and DFT studies have been used to confirm the binding sites of 5HPD with Cit.•This sensor is highly reversible for three cycles with a very good RSD in real sample analysis.•Further, it was applied for logic gate circuit. A new curcumin-based biosensor (E)-5-(4-hydroxyphenyl)-1-phenylpent-4-ene-1,3‑dione (5HPD) was successfully synthesized for the selective detection of citrulline (Cit). Job's plot analysis indicated, a 1:1 binding stoichiometry between the sensor (5HPD) and citrulline. The binding constant was estimated to be 3.55  × 107 M−1 according to the Stern Volmer method. The newly synthesized sensor 5HPD is highly selective detection towards citrulline with various competitive biomolecules. Linearity ranges of 10 - 60 μM and lower detection limits is 75 nM. Interestingly, a reversible process provoke successively by 5HPD, Cit with EDTA observed and molecular logic circuits. The Mechanism of sensing 5HPD-Cit has been characterized clearly by 1H NMR, FTIR, ESI-MS spectroscopy and DFT calculation. [Display omitted]