The mechanism of action of limonoids of Citri Reticulatae Semen against MCF-7 breast cancer cells

•Limonin has good anti-breast cancer effects and development prospects.•We identified potential biomarkers of Limonin against breast cancer, and it play an important role in the prognosis and progression of breast cancer.•Limonin can exert anti-breast cancer effects by inhibiting the proliferation o...

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Bibliographic Details
Published in:Journal of molecular structure 2024-02, Vol.1298, p.137050, Article 137050
Main Authors: Huang, Xulong, Jiang, Huajuan, Wang, Yue, Shuai, Xiaomin, Wan, Xuanting, Pei, Jin, Wu, Qinghua
Format: Article
Language:English
Subjects:
Apoptosis and cycle
Bioinformatics
Biomarker
Breast cancer cell
Limonoids
Mechanisms
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Summary:•Limonin has good anti-breast cancer effects and development prospects.•We identified potential biomarkers of Limonin against breast cancer, and it play an important role in the prognosis and progression of breast cancer.•Limonin can exert anti-breast cancer effects by inhibiting the proliferation of MCF-7 cells, down-regulating the expression levels of this proteins, inducing cell arrest in the G2-M phase, promoting apoptosis, and regulating the p53 signaling pathway. Limonoids (LMD), particularly limonin, possess significant anti-breast cancer (BC) potential. However, their precise anti- breast cancer mechanisms remain unelucidated. In this study, we aimed to identify potential biomarkers associated with BC development and metastasis and to investigate LMD anti-BC activity. Key LMD components were analyzed using UPLC-QQQ-MS/MS, with various bioinformatics tools utilized for additional analysis. These included ADME and toxicity prediction, Weighted Gene Co-expression Network Analysis (WGCNA), and construction of protein-protein interaction (PPI) and “LMD-Hub-gene-BC” models. A total of 1898 DEGs were obtained including 622 up-regulated and 1276 down-regulated genes. The WGCNA identified 4 significant modules. The 11 hub genes were identified by NTA. The results of enrichment analysis showed that 631 significant GO conditions and 34 significant KEGG pathways were enriched, of which cell cycle, p53 signaling pathway and Prostate cancer might be the main regulatory pathways for its anti-BC. The gene expression and immunohistochemistry of the result suggests that most of the hub genes expression has significant differences (P 
ISSN:0022-2860
1872-8014
DOI:10.1016/j.molstruc.2023.137050