Synthesis and biomedical potential of silk fiber/methylcellulose composite containing copper and zinc co-substituted hydroxyapatite for bone tissue engineering applications

• Published in: Journal of molecular structure 2025-02, Vol.1321, p.139922, Article 139922
• Main Authors: Narayanan, Valarmathi, Shanmugam, Sumathi
• Format: Article
• Language: English
• Subjects: Bioactivity; Copper zinc hydroxyapatite; In-vitro hemocompatibility; Methylcellulose; Silk fiber
• ISSN: 0022-2860
• DOI: 10.1016/j.molstruc.2024.139922

•Fabricated nanocomposite of Silk fiber/Methylcellulose (SF/MC) with different weight percentage of (10, 20, 30, 40 and 50 wt %) of copper and zinc co-substituted hydroxyapatite nanocomposites were prepared by E-Spin method.•Increasing the concentration of copper and zinc in hydroxyapatite and their wt % (10–40 wt %) into the SF/MC nanocomposite increased the porosity and tensile strength.•The optimized nanocomposite (40 wt % of Cu1.5 into the SF/MC) enhanced the biocompatibility and the new apatite layer was formed on surface of the nanocomposite. The primary feature of biomaterials is to aid in the regeneration of damaged tissues and organs. To attain a biomaterial, we fabricated a double mineral (copper and zinc) substituted hydroxyapatite/silk fiber/methylcellulose (CZ-HAP/SF/MC) nanocomposite by the E-Spin method. The as-developed CZ-HAP/SF/MC nanocomposite was characterized by Fourier transform infrared spectroscopy (FT-IR), X-ray diffraction (XRD) and moreover, the fabricated nanocomposites were used to investigate morphological changes, porosity, swelling test, mechanical strength, antibacterial activity and apatite growth. In addition, antimicrobial studies demonstrated that CZ-HAP/SF/MC nanocomposite exhibited excellent antimicrobial activity against S. aureus, E. coli and C. albicans and, the resultant nanocomposite revealed that the CZ-HAP/SF/MC nanocomposite will be an effective implant material for better cell growth in BTE applications. [Display omitted]