GSTP1 and XRCC1 polymorphisms and DNA damage in agricultural workers exposed to pesticides

•Prevalence of genotypes of metabolic GSTP1 was studied.•Prevalence of genotypes of DNA-repair XRCC1 was studied.•DNA damage was estimated by comet assay.•Correlations of genotypes with DNA damage were estimated in pesticide sprayers.•Results can be applied to monitor risks among agricultural worker...

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Bibliographic Details
Published in:Mutation research. Genetic toxicology and environmental mutagenesis 2017-07, Vol.819, p.20-25
Main Authors: Saad-Hussein, Amal, Noshy, Magda, Taha, Mona, El-Shorbagy, Haidan, Shahy, Eman, Abdel-Shafy, Ebtesam A.
Format: Article
Language:English
Subjects:
Comet assay
GSTP1
Pesticide exposure
Polymorphisms
XRCC1
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Summary:•Prevalence of genotypes of metabolic GSTP1 was studied.•Prevalence of genotypes of DNA-repair XRCC1 was studied.•DNA damage was estimated by comet assay.•Correlations of genotypes with DNA damage were estimated in pesticide sprayers.•Results can be applied to monitor risks among agricultural workers. Pesticide exposure may be associated with increased risk of genotoxicity and carcinogenesis. These risks may be affected by polymorphisms of genes for glutathione transferase-dependent metabolism of pesticides and for DNA repair. We studied the prevalence of GSTP1 and XRCC1 polymorphisms and their possible correlation with DNA damage following prolonged pesticide exposure. DNA damage was estimated by the comet assay in peripheral blood samples from 51 pesticide-exposed workers and 50 controls. GSTP1 (105) and XRCC1 (399 and 194) genotypes were identified by restriction fragment length analysis. Individuals carrying theGSTP1 Ile-Ile or XRCC1399 Arg-Arg genotypes showed greater DNA damage than observed for other alleles.
ISSN:1383-5718
1879-3592
DOI:10.1016/j.mrgentox.2017.05.005