Synthesis of 1,4-diazabicyclo[2.2.2]octane and pyridinium based cationic polymers via ROMP technique and examination of their antibacterial activity and cytotoxicity

• Published in: Materialia 2019-03, Vol.5, p.100246, Article 100246
• Main Authors: Kaymaz, Aylin Pak, Acaroğlu-Degitz, İlayda, Yapaöz, Melda Altıkatoğlu, Sezer, Ali Demir, Malta, Seyda, Aksu, Burak, Eren, Tarik
• Format: Article
• Language: English
• Subjects: Antimicrobial activity; Cationic polymer; DABCO; Hemolytic concentration; Pyridinium; ROMP
• ISSN: 2589-1529; 2589-1529
• DOI: 10.1016/j.mtla.2019.100246

This paper focuses on the synthesis of cationic antibacterial polymers that could be a potential source for new-generation antibiotics with well-defined architecture derived from the Ring Opening Metathesis Polymerization (ROMP) technique. Mono- and double-charge bearing quaternary groups have been used to synthesize cationic homopolymers (MWs: 3000 and 10,000 g/mole) and their copolymers (MWs: 5000 g/mole). Hemolytic concentration (HC50, ≥1000 µg/mL) and MTS assay results showed that the polymers are non-toxic. It has been observed that the double-charge bearing polymers have the highest antimicrobial activity (S. aureus= 8 µg/mL) and a high selectivity against S. aureus (>250). Percent killing efficiencies were tested on a glass surface where moderate killing efficiency was observed in the range of 40–80% in 5 min. Cationic charge density and zeta potential studies were used to investigate the mechanisms of antimicrobial efficiency of the polymers in a solution during the action against S. aureus to understand structure-activity relationships. Scanning electron microscopy (SEM) was also conducted for the bacterial morphology assay. [Display omitted]