Theranostic doxorubicin encapsulated FeAu alloy@metal-organic framework nanostructures enable magnetic hyperthermia and medical imaging in oral carcinoma

• Published in: Nanomedicine 2023-02, Vol.48, p.102652, Article 102652
• Main Authors: Dhawan, Udesh, Tseng, Ching-Li, Wu, Ping-Hsuan, Liao, Mei-Yi, Wang, Huey-Yuan, Wu, Kevin C.-W., Chung, Ren-Jei
• Format: Article
• Language: English
• Subjects: Animals; cancer theranostics; Carcinoma; Diagnostic Imaging; Doxorubicin - chemistry; Doxorubicin - pharmacology; Doxorubicin - therapeutic use; Hyperthermia; Hyperthermia, Induced; iron‑gold alloy nanoparticles; Magnetic Phenomena; Metal-organic framework; Metal-Organic Frameworks - chemistry; Mice; Mouth Neoplasms - diagnostic imaging; Mouth Neoplasms - drug therapy; Nanostructures; Oral squamous carcinoma; Precision Medicine; Theranostic Nanomedicine
• ISSN: 1549-9634; 1549-9642
• DOI: 10.1016/j.nano.2023.102652

Metal-organic frameworks (MOFs) have emerged as attractive candidates in cancer theranostics due to their ability to envelop magnetic nanoparticles, resulting in reduced cytotoxicity and high porosity, enabling chemodrug encapsulation. Here, FeAu alloy nanoparticles (FeAu NPs) are synthesized and coated with MIL-100(Fe) MOFs to fabricate FeAu@MOF nanostructures. We encapsulated Doxorubicin within the nanostructures and evaluated the suitability of this platform for medical imaging and cancer theranostics. FeAu@MOF nanostructures (FeAu@MIL-100(Fe)) exhibited superparamagnetism, magnetic hyperthermia behavior and displayed DOX encapsulation and release efficiency of 69.95 % and 97.19 %, respectively, when stimulated with alternating magnetic field (AMF). In-vitro experiments showed that AMF-induced hyperthermia resulted in 90 % HSC-3 oral squamous carcinoma cell death, indicating application in cancer theranostics. Finally, in an in-vivo mouse model, FeAu@MOF nanostructures improved image contrast, reduced tumor volume by 30-fold and tumor weight by 10-fold, which translated to enhancement in cumulative survival, highlighting the prospect of this platform for oral cancer treatment. Metal-organic framework-FeAu NPs (MOF-FeAu) nanostructures are fabricated for hyperthermia-induced cancer treatment and imaging. Different shells of MOF were synthesized using cysteine as a linker. An increase in MOF shell number increased biocompatibility and enhanced chemodrug (doxorubicin) encapsulation. In an in-vivo mouse model, Doxorubicin-loaded MOF-FeAu nanostructures displayed drastic reduction in tumor size, elevation in survival rate which assistance in tumor imaging. [Display omitted] •Metal-organic framework-decorated iron-gold alloy nanoparticles were synthesized.•The nanostructures enabled encapsulation of anti-cancer drug, doxorubicin.•Upon magnetic field stimulation, the nanostructures generated hyperthermia.•Hyperthermia-induced doxorubicin release triggered apoptosis of oral cancer cells.•The platform can be utilized for imaging and it suppressed tumor growth in-vivo.