Astrocytic transactivation by α2A-adrenergic and 5-HT2B serotonergic signaling

• Published in: Neurochemistry international 2010-11, Vol.57 (4), p.421-431
• Main Authors: Peng, Liang, Li, Baoman, Du, Ting, Kong, Ebenezer K.C., Hu, Xiaoling, Zhang, Shiquen, Shan, Xiaolei, Zhang, Meixia
• Format: Article
• Language: English
• Subjects: 5-HT2B receptor; Astrocytes; Biological and medical sciences; EGF receptor; ERK; Fluoxetine; Fundamental and applied biological sciences. Psychology; Vertebrates: nervous system and sense organs; α2A-Adrenergic receptor
• ISSN: 0197-0186; 1872-9754
• DOI: 10.1016/j.neuint.2010.04.018

EGF receptor transactivation has been known for more than ten years. It is a signal pathway in which a G-protein-coupled receptor (GPCR) signal leads to release of a growth factor, which in turn activates the EGF receptor-tyrosine kinase in the same or adjacent cells. Astrocytes express a number of GPCRs and play key roles in brain function. Astrocytic transactivation is of special interest, since its autocrine effect may regulate gene expression and alter cell functions in the cells themselves and its paracrine effect may provide additional opportunities for cross-talk between astrocytes and their neighbors, such as neurons. The signal pathways of EGF transactivation are complicated. This does not only apply to the pathways leading to shedding of growth factor(s), but also to the downstream signal pathways of the EGF receptor, i.e., MAPK and PI3K. The latter may vary according to the type of growth factor released, the sites of tyrosine phosphorylation on the EGF receptor, and the duration of the phosphorylation. Using primary cell cultures we have found that dexmedetomidine, a specific α2-adrenergic receptor, induced shedding of HB-EGF from astrocytes, which in turn transactivated EGF receptors and stimulated astrocytic c-Fos and FosB expression. At the same time released HB-EGF protected neurons from injury caused by H2O2. We have also confirmed dexmedetomidine transactivation in the brain in vivo. EGF transactivation by 5-HT2B receptor stimulation was responsible for up-regulation of cPLA2 in astrocytes by fluoxetine, an antidepressant and inhibitor of the serotonin transporter, which also is a specific 5-HT2B agonist.