Status epilepticus induced by pilocarpine and Ca2+ transport by microsome in the hippocampus of rats

An increase in intra-neuronal Ca(2+) concentration has been associated to status epilepticus (SE). Ca(2+) is stored in the endoplasmic reticulum, mediated by the Ca(2+)-ATPases (SERCAs). Here we studied the Ca(2+)-ATPase activity and the SERCA2b distribution in the hippocampus of rats submitted to 5...

Full description

Saved in:
Bibliographic Details
Published in:Neuroscience letters 2004-08, Vol.366 (3), p.292-296
Main Authors: COSTA, Maricilia S, FUNKE, Marcelo G, DE MEIS, Leopoldo, CAVALHEIRO, Esper A, NAFFAH-MAZZACORATTI, Maria Da
Format: Article
Language:English
Subjects:
Adenosine Triphosphate - metabolism
Analysis of Variance
Animals
Biological and medical sciences
Biological Transport - physiology
Calcium - metabolism
Calcium - pharmacology
Calcium-Transporting ATPases - metabolism
Disease Models, Animal
Dose-Response Relationship, Drug
Fundamental and applied biological sciences. Psychology
Headache. Facial pains. Syncopes. Epilepsia. Intracranial hypertension. Brain oedema. Cerebral palsy
Hippocampus - metabolism
Immunohistochemistry - methods
Male
Medical sciences
Microsomes - metabolism
Mixed Function Oxygenases
Nervous system (semeiology, syndromes)
Neurology
Pilocarpine
Rats
Rats, Wistar
Status Epilepticus - chemically induced
Status Epilepticus - metabolism
Time Factors
Vertebrates: nervous system and sense organs
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:An increase in intra-neuronal Ca(2+) concentration has been associated to status epilepticus (SE). Ca(2+) is stored in the endoplasmic reticulum, mediated by the Ca(2+)-ATPases (SERCAs). Here we studied the Ca(2+)-ATPase activity and the SERCA2b distribution in the hippocampus of rats submitted to 5h of SE. The Ca(2+)-uptake was measured using [45Ca]CaCl(2) and the hippocampal distribution of SERCA2b was analyzed by immunohistochemistry. A reduction in the amount of cells expressing SERCA2b in CA1, CA3 and dentate gyrus was observed. However, the surviving cells of these regions increased the SERCA2b immunoreactivity, when compared with control tissues. The Ca(2+)-ATPase activity measured in all hippocampal formation was not modified by SE. These results suggest that SE promotes a redistribution of SERCA2b in the hippocampus as a compensatory Ca(2+)-transport mechanism.
ISSN:0304-3940
1872-7972
DOI:10.1016/j.neulet.2004.05.052