Analytical possibilities of highly focused ion beams in biomedical field

• Published in: Nuclear instruments & methods in physics research. Section B, Beam interactions with materials and atoms Beam interactions with materials and atoms, 2017-09, Vol.406, p.15-24
• Main Authors: Ren, M.Q., Ji, X, Vajandar, S.K., Mi, Z.H., Hoi, A., Walczyk, T, van Kan, J.A., Bettiol, A.A., Watt, F., Osipowicz, T.
• Format: Article
• Language: English
• Subjects: Diseases; Focused ion beam; PIXE; RBS; STIM; Whole cell imaging
• ISSN: 0168-583X; 1872-9584
• DOI: 10.1016/j.nimb.2017.01.004

At the Centre for Ion Beam Applications (CIBA), a 3.5MV HVEE Singletron™ accelerator serves to provide MeV ion beams (mostly protons or He+) to six state-of-the-art beam lines, four of which are equipped with Oxford triplet magnetic quadrupole lens systems. This facility is used for a wide range of research projects, many of which are in the field of biomedicine. Here we presented a discussion of currently ongoing biomedical work carried out using two beamlines:(a)The Nuclear Microscopy (NM) beamline is mainly used for trace elemental quantitative mapping using a combination of Particle Induced X-ray Emission (PIXE), to measure the trace elemental concentration of inorganic elements, Rutherford Backscattering Spectrometry (RBS), to characterise the organic matrix, and Scanning Transmission Ion Microscopy (STIM) to provide information on the lateral areal density variations of the specimen. Typically, a 2.1MeV proton beam, focused to 1–2μm spot size with a current of 100pA is used.(b)The high resolution single cell imaging beamline is equipped with direct STIM to image the interior structure of single cells with proton and alpha particles of sub-50nm beam spot sizes. Simultaneously, forward scattering transmission ion microscopy (FSTIM) is utilized to generate images with improved contrast of nanoparticles with higher atomic numbers, such as gold nanoparticles, and fluorescent nanoparticles can be imaged using Proton Induced Fluorescence (PIF). Lastly, in this facility, RBS has been included as an option if required to determine the depth distribution of nanoparticles in cells, albeit with reduced spatial resolution.