Diffusion kinetics of the glucose/glucose oxidase system in swift heavy ion track-based biosensors

• Published in: Nuclear instruments & methods in physics research. Section B, Beam interactions with materials and atoms Beam interactions with materials and atoms, 2017-05, Vol.398, p.21-26
• Main Authors: Fink, Dietmar, Vacik, Jiri, Hnatowicz, V., Muñoz Hernandez, G., Garcia Arrelano, H., Alfonta, Lital, Kiv, Arik
• Format: Article
• Language: English
• Subjects: Biosensor; Diffusion; Enzyme; Etched ion tracks; Polymer; Track radius
• ISSN: 0168-583X; 1872-9584
• DOI: 10.1016/j.nimb.2017.03.050

•Application of swift heavy ion tracks in biosensing.•Obtaining yet unknown diffusion coefficients of organic matter across etched ion tracks.•Obtaining diffusion coefficients of organics in etched ion tracks of biosensors.•Comparison with Renkin’s equation to predict the effective etched track diameter in the given experiments. For understanding of the diffusion kinetics and their optimization in swift heavy ion track-based biosensors, recently a diffusion simulation was performed. This simulation aimed at yielding the degree of enrichment of the enzymatic reaction products in the highly confined space of the etched ion tracks. A bunch of curves was obtained for the description of such sensors that depend only on the ratio of the diffusion coefficient of the products to that of the analyte within the tracks. As hitherto none of these two diffusion coefficients is accurately known, the present work was undertaken. The results of this paper allow one to quantify the previous simulation and hence yield realistic predictions of glucose-based biosensors. At this occasion, also the influence of the etched track radius on the diffusion coefficients was measured and compared with earlier prediction.