External RBS/PIXE analysis for evaluating quantum dots internalization into HeLa cells

We have developed an external RBS/PIXE setup at University of Göttingen. By using this setup, the analysis can be performed in atmospheric condition, which leads to realizing a quantitative analysis for volatile samples with depth information. We applied this technique for HeLa cells, internalized C...

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Bibliographic Details
Published in:Nuclear instruments & methods in physics research. Section B, Beam interactions with materials and atoms Beam interactions with materials and atoms, 2019-07, Vol.450, p.173-178
Main Authors: Saito, M., Koike, S., Holm, K., Bregolin, F.L., Hofsäss, H.
Format: Article
Language:English
Subjects:
External RBS
HeLa cells
Internalization
Quantum dot
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Summary:We have developed an external RBS/PIXE setup at University of Göttingen. By using this setup, the analysis can be performed in atmospheric condition, which leads to realizing a quantitative analysis for volatile samples with depth information. We applied this technique for HeLa cells, internalized CdSe quantum dots (Q-dot). External RBS shows the depth difference of Q-dot depth distribution in cells, w or w/o the treatment with an endocytosis inhibitor, which agrees well with the result of confocal microscopy. The quantity of Q-dot is also evaluated by external PIXE, which coincides with the result of RBS and fluorescence measurement. In addition, the application of Si3N4 film as a medium for HeLa cells is presented.
ISSN:0168-583X
1872-9584
DOI:10.1016/j.nimb.2018.08.027