Quantitative nitric oxide production by rat, bovine and porcine macrophages

The aim of this work was to compare in vitro nitric oxide (NO) production by rat, bovine and porcine macrophages. NO production was induced by lipopolysaccharide (LPS) or by phorbol 12-myristate 13-acetate (PMA) with ionomycin or recombinant interferon gamma (rIFN-γ) and was assessed by Griess react...

Full description

Saved in:
Bibliographic Details
Published in:Nitric oxide 2008-08, Vol.19 (1), p.36-41
Main Authors: Zelnickova, Petra, Matiasovic, Jan, Pavlova, Barbora, Kudlackova, Hana, Kovaru, Frantisek, Faldyna, Martin
Format: Article
Language:English
Subjects:
Animals
Biological Assay
Blotting, Western
Cattle
Cells, Cultured
Cow
Ethylenediamines
Gene Expression Regulation, Enzymologic
Immunohistochemistry
Interferon-gamma - toxicity
Ionomycin - toxicity
Kupffer cells
Lipopolysaccharides - toxicity
Macrophages, Alveolar - metabolism
Macrophages, Alveolar - pathology
Nitric oxide
Nitric Oxide - analysis
Nitric Oxide - metabolism
Nitric oxide synthase
Nitric Oxide Synthase Type II - genetics
Nitric Oxide Synthase Type II - metabolism
Pig
Pulmonary alveolar macrophages
Rat
Rats
Recombinant Proteins
Reverse Transcriptase Polymerase Chain Reaction
RNA, Messenger - metabolism
Sulfanilamides
Swine
Tetradecanoylphorbol Acetate - toxicity
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:The aim of this work was to compare in vitro nitric oxide (NO) production by rat, bovine and porcine macrophages. NO production was induced by lipopolysaccharide (LPS) or by phorbol 12-myristate 13-acetate (PMA) with ionomycin or recombinant interferon gamma (rIFN-γ) and was assessed by Griess reaction. NO synthase type II (NOS II) expression was quantified by immunocytochemistry, Western blot and real-time polymerase chain reaction (RT-PCR). There were differences in NO production by pulmonary alveolar macrophages (PAM) in all species tested. The largest amounts of NO were produced by rat PAM. Less NO was produced by bovine PAM. Moreover, PAM in rats and cows differed in their abilities to respond to various stimulators. Neither porcine PAM nor Kupffer cells produced NO. Stimulation of porcine PAM with alternative concentrations of LPS did not lead to inducing NO production. Stimulation of porcine PAM with rIFN-γ together with LPS led to a significant increase in the expression of NOS II mRNA, albeit without detectable NO production or NOS II expression on the protein level.
ISSN:1089-8603
1089-8611
DOI:10.1016/j.niox.2008.04.001