A single 30min treadmill exercise session is suitable for ‘proof-of concept studies’ in adult mdx mice: A comparison of the early consequences of two different treadmill protocols

The extent of muscle pathology in sedentary adult mdx mice is very low and treadmill exercise is often used to increase myofibre necrosis; however, the early events in dystrophic muscle and blood in response to treadmill exercise (leading to myofibre necrosis) are unknown. This study describes in de...

Full description

Saved in:
Bibliographic Details
Published in:Neuromuscular disorders : NMD 2012-02, Vol.22 (2), p.170-182
Main Authors: Radley-Crabb, Hannah, Terrill, Jessica, Shavlakadze, Thea, Tonkin, Joanne, Arthur, Peter, Grounds, Miranda
Format: Article
Language:English
Subjects:
Creatine kinase
Inflammation
Mdx mouse
Myofibre necrosis
Oxidative stress
Skeletal muscle damage
Treadmill exercise
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:The extent of muscle pathology in sedentary adult mdx mice is very low and treadmill exercise is often used to increase myofibre necrosis; however, the early events in dystrophic muscle and blood in response to treadmill exercise (leading to myofibre necrosis) are unknown. This study describes in detail two standardised protocols for the treadmill exercise of mdx mice and profiles changes in molecular and cellular events after a single 30min treadmill session (Protocol A) or after 4weeks of (twice weekly) treadmill exercise (Protocol B). Both treadmill protocols increased multiple markers of muscle damage. We conclude that a single 30min treadmill exercise session is a sufficient and conveniently fast screening test and could be used in ‘proof-of-concept’ studies to evaluate the benefits of pre-clinical drugs in vivo. Myofibre necrosis, blood serum CK and oxidative stress (specifically the ratio of oxidised to reduced protein thiols) are reliable markers of muscle damage after exercise; many parameters demonstrated high biological variation including changes in mRNA levels for key inflammatory cytokines in muscle. The sampling (sacrifice and tissue collection) time after exercise for these parameters is critical. A more precise understanding of the changes in dystrophic muscle after exercise aims to identify biomarkers and new potential therapeutic drug targets for Duchenne Muscular Dystrophy.
ISSN:0960-8966
1873-2364
DOI:10.1016/j.nmd.2011.07.008