[18F]FDG and [11C]PK11195 PET imaging in the evaluation of brown adipose tissue - effects of cold and pharmacological stimuli and their association with crotamine intake in a male mouse model

• Published in: Nuclear medicine and biology 2023-07, Vol.122-123, p.108362, Article 108362
• Main Authors: de Paula Faria, Daniele, D'Arc Campeiro, Joana, de Souza Junqueira, Mara, Real, Caroline Cristiano, Marques, Fabio Luiz Navarro, Hayashi, Mirian Akemi Furuie, Sapienza, Marcelo Tatit
• Format: Article
• Language: English
• Subjects: [11C]PK11195; [18F]FDG; Adipose Tissue, Brown - diagnostic imaging; Adrenergic Agents - metabolism; Animals; Brown adipose tissue; Crotalid venoms; Fluorodeoxyglucose F18 - metabolism; Male; Mice; Mice, Inbred C57BL; Positron emission tomography; Positron-Emission Tomography - methods
• ISSN: 0969-8051; 1872-9614
• DOI: 10.1016/j.nucmedbio.2023.108362

This study aimed to evaluate the role of positron emission tomography (PET) with [11C]PK11195 and [18F]FDG in the characterization of brown adipose tissue (BAT). Male C57BL/6 mice were studied with the glucose analogue [18F]FDG (n = 21) and the TSPO mitochondrial tracer [11C]PK11195 (n = 28), without stimulus and after cold (6–9 °C) or beta-agonist (CL316243) stimuli. PET studies were performed at baseline and after 21 days of daily treatment with crotamine, which is a peptide described to induce adipocyte tissue browning and to increase BAT metabolism. Tracer uptake (SUVmax) was measured in the interscapular BAT and translocator protein 18 kDa (TSPO) expression was evaluated by immunohistochemistry. The cold stimulus increased [18F]FDG uptake compared to no-stimulus (5.21 ± 1.05 vs. 2.03 ± 0.21, p