Human erythrocytes as a system for evaluating the antioxidant capacity of vegetable extracts

• Published in: Nutrition research (New York, N.Y.) N.Y.), 2008-07, Vol.28 (7), p.457-463
• Main Authors: Arbos, Kettelin A, Claro, Ligia M, Borges, Lucielly, Santos, Cid A.M, Weffort-Santos, Almeriane M
• Format: Article
• Language: English
• Subjects: antioxidant activity; Antioxidants; Antioxidants - pharmacology; Biological and medical sciences; Biphenyl Compounds; Brassica - chemistry; Brassicaceae; Brassicaceae - chemistry; broccoli; cell culture; Erythrocytes - chemistry; Erythrocytes - drug effects; Feeding. Feeding behavior; free radical scavengers; Free Radical Scavengers - pharmacology; Free radicals; Fundamental and applied biological sciences. Psychology; Gastroenterology and Hepatology; glutathione; health promotion; hemoglobin; Human erythrocytes; Humans; kale; Methemoglobin; Methemoglobin - analysis; methemohemoglobin; Oxidants - pharmacology; Oxidative Stress; Phenylhydrazines - pharmacology; phytochemicals; Picrates; Plant Extracts - pharmacology; polyphenols; radishes; Raphanus - chemistry; reactive oxygen species; reticulocytes; vegetable extracts; Vegetables; Vertebrates: anatomy and physiology, studies on body, several organs or systems
• ISSN: 0271-5317; 1879-0739
• DOI: 10.1016/j.nutres.2008.04.004

Free radicals are fairly unstable and highly reactive substances, able of causing oxidation and sometimes-irreversible damage to cells, compromising their function. The Brassicaceae family has many important species for the regular human diet as they provide several antioxidant constituents. In this study, the antioxidant potential of the hydroethanolic extracts prepared from the edible parts of kale, broccoli, and radish was investigated in vitro using human erythrocytes under oxidative stress imposed by phenylhydrazine as an experimental model, in which the methemoglobin levels were measured. When the results were compared with the antioxidant capacity shown by the traditional 2,2-diphenyl-2-picrylhydrazyl hydrate free radical and phosphomolybdenum complex methods, the extracts tested showed significant and correspondent antioxidant activity. Broccoli extract presented the highest antioxidant activity, followed closely by the kale, whereas the radish extract occupied the lowest position. The results derived from the human erythrocyte system have shown it as an alternative method for evaluating the antioxidant properties of vegetable extracts.