Association of genetic polymorphism of interleukin 1-alpha and interleukin 1-beta with external apical root resorption in orthodontic patients

• Published in: International orthodontics 2024-06, Vol.22 (2), p.100869, Article 100869
• Main Authors: Chauhan, Jyoti, Sharma, Payal, Shetty, Devicharan, Kumar, Piush, Jain, Shubhangi, Suroliya, Varun, Garg, Prince
• Format: Article
• Language: English
• Subjects: Gene polymorphism; Genetics; Interleukins; Orthodontics; PCR; Root resorption
• ISSN: 1761-7227
• DOI: 10.1016/j.ortho.2024.100869

To assess the allele rs 1143634 in IL-1β and rs1800587 in IL-1α in patients for orthodontically induced external apical root resorption (EARR). Intra-oral periapical radiograph (IOPA) of maxillary incisors of 142 Patients were evaluated for resorption at two time points; before the start of fixed mechanotherapy (T1) and after one year of treatment (T2). The individuals with root resorption2mm were categorized as case group (group 2; n=52). Buccal swabs of all patients were taken and DNA could be isolated in 95 out of 142 samples (group 1 {n=58}, group 2 {n=37}), which were then screened for the selected two polymorphic targets to determine the nucleotide status of these targets. Tetra-primer ARMS PCR reactions were carried out using all 4 primers for each polymorphism. rs11800587 was not associated with risk of EARR in any inheritance model. Chi-square test for association of alleles with EARR revealed that rs1143634 was associated with the risk of EARR in an allelic model in such a way that A allele of this SNP increased the risk of EARR 4 folds [OR=4.375; P=0.016]. However, the adjusted level of significance using the Holm-Bonferroni method for rs1143634 was P