The Impact of a Cation Channel Blocker (Furosemide) on Pseudomonas aeruginosa PA01 Biofilm Architecture

OBJECTIVE: To determine if furosemide could have a role in inhibition of biofilm formation. STUDY DESIGN: A prospective study of 72-hour biofilms grown in the Calgary Biofilm Device (CBD) treated with furosemide and controls. METHODS: Bacteria were treated with furosemide and controls to determine i...

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Published in:Otolaryngology-head and neck surgery 2007-07, Vol.137 (1), p.21-26
Main Authors: Cross, Jenny L., Ramadan, Hassan H., Thomas, John G.
Format: Article
Language:English
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Summary:OBJECTIVE: To determine if furosemide could have a role in inhibition of biofilm formation. STUDY DESIGN: A prospective study of 72-hour biofilms grown in the Calgary Biofilm Device (CBD) treated with furosemide and controls. METHODS: Bacteria were treated with furosemide and controls to determine its antiplanktonic properties. PA01 biofilms were treated with serial 2-fold dilutions of furosemide and controls over a 48-hour period at pH concentrations of 7.0, 8.0, and 9.0. RESULTS: Furosemide had no effect on planktonic PA01. It had minimal effect at pH below 8.0. A pH of 8.0 and 9.0 reduced biofilms 25%. Furosemide 10 mg/mL reduced biofilms 50% at pH 8.0 and 9.0. It inhibited biofilms to
ISSN:0194-5998
1097-6817
DOI:10.1016/j.otohns.2007.02.022