Characterization and optimization of vascular endothelial growth factor165 (rhVEGF165) expression in Escherichia coli

► l-Arabinose (0.25% w/v) at 20°C for 5h provided optimal culture conditions for rhVEGF165 expression by Escherichia coli. ► Among culture conditions, induction temperature greatly influences the rhVEGF165 expression. ► rhVEGF165 significantly increased cell proliferative activity (p

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Bibliographic Details
Published in:Protein expression and purification 2013-02, Vol.87 (2), p.55-60
Main Authors: Kang, W., Kim, S., Lee, S., Jeon, E., Lee, Y., Yun, Y.R., Suh, C.K., Kim, H.W., Jang, J.H.
Format: Article
Language:English
Subjects:
Calf pulmonary artery endothelial cell
Cell proliferative activity
Gene expression
Protein expression
Vascular endothelial growth factor
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Summary:► l-Arabinose (0.25% w/v) at 20°C for 5h provided optimal culture conditions for rhVEGF165 expression by Escherichia coli. ► Among culture conditions, induction temperature greatly influences the rhVEGF165 expression. ► rhVEGF165 significantly increased cell proliferative activity (p
ISSN:1046-5928
1096-0279
DOI:10.1016/j.pep.2012.10.004