Development of a biochemical and biophysical suite for integral membrane protein targets: A review

The generation of integral membrane proteins (IMPs) in heterologous systems and their characterization remains a major challenge in biomedical research. Significant efforts have been invested both in academia and in the pharmaceutical industry to establish technologies for the expression, isolation...

Full description

Saved in:
Bibliographic Details
Published in:Protein expression and purification 2020-03, Vol.167, p.105545, Article 105545
Main Authors: Haffke, Matthias, Duckely, Myriam, Bergsdorf, Christian, Jaakola, Veli-Pekka, Shrestha, Binesh
Format: Article
Language:English
Subjects:
Animals
Antibodies
Baculoviridae - genetics
Biophysics
Cell Line
Drug Design
Drug Industry
Expression
G protein coupled receptors
Gene Expression
GPCR
Humans
Insect cells
Insecta - genetics
Integral membrane proteins
Mammalian cells
Mammals - genetics
Membrane Proteins - biosynthesis
Membrane Proteins - chemistry
Membrane Proteins - immunology
Membrane Proteins - isolation & purification
Receptors, G-Protein-Coupled - biosynthesis
Receptors, G-Protein-Coupled - chemistry
Receptors, G-Protein-Coupled - immunology
Receptors, G-Protein-Coupled - isolation & purification
Recombinant Proteins - biosynthesis
Recombinant Proteins - chemistry
Recombinant Proteins - immunology
Recombinant Proteins - isolation & purification
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:The generation of integral membrane proteins (IMPs) in heterologous systems and their characterization remains a major challenge in biomedical research. Significant efforts have been invested both in academia and in the pharmaceutical industry to establish technologies for the expression, isolation and characterization of IMPs. Here we summarize some of the key aspects, which are important to support structure-based drug design (SBDD) in drug discovery projects. We furthermore include timeline estimates and an overview of the target selection and biophysical screening approaches. •Integral membrane protein (IMP) production in eukaryotic expression systems (insect cells and mammalian cells).•Key technological aspects in expression, isolation and characterization of IMPs.•Focused on IMP tool generation to support structure-based drug design (SBDD).•Methods and tool suitable for experienced scientists as well as for beginners in the field.
ISSN:1046-5928
1096-0279
DOI:10.1016/j.pep.2019.105545