Isolation and characterization of a novel glutathione S-transferase-activating peptide from the oriental medicinal plant Phellodendron amurense

• Published in: Peptides (New York, N.Y. : 1980) N.Y. : 1980), 2006-09, Vol.27 (9), p.2069-2074
• Main Authors: Lee, Ju Hyun, Lee, Dae Hyoung, Yu, Hyung Eun, Kim, Jae Ho, Lee, Jong Soo
• Format: Article
• Language: English
• Subjects: Animals; Biological and medical sciences; Dinitrochlorobenzene - pharmacology; Dinitrochlorobenzene - toxicity; Fundamental and applied biological sciences. Psychology; Glutathione S-transferase-activating peptide; Glutathione Transferase - metabolism; Korea; Male; Mice; Mice, Inbred ICR; Oligopeptides - chemistry; Oligopeptides - isolation & purification; Oligopeptides - metabolism; Oligopeptides - pharmacology; Oriental medicinal plant; Phellodendron - chemistry; Phellodendron amurense; Plant Extracts - chemistry; Plant Extracts - pharmacology; Plants, Medicinal - chemistry; Plants, Medicinal - cytology; Vertebrates: endocrinology
• ISSN: 0196-9781; 1873-5169
• DOI: 10.1016/j.peptides.2006.03.004

The aim of this study was to elucidate the characteristics of glutathione S-transferase(GST)-activating compounds from medicinal plants. Among 265 kinds of medicinal plants, Phellodendron amurense showed the highest GST activity at 174.8%. The GST-activating compound of P. amurense was maximally extracted when treated with distilled water at 30 °C for 12 h. The compound was purified by ultrafiltration, Sephadex G-10 gel filtration chromatography, and reverse-phase HPLC. The purified GST-activating compound from P. amurense was a novel tetrapeptide with an amino acid sequence of Ala-Pro-Trp-Cys and its molecular weight was estimated to be 476 Da. It also displayed a clear detoxicative effect in 1-chloro-2,4-dinitrobenzene treated mice at a dosage of mg/kg body weight.