A novel immobilized cholinesterase for on-site screening of organophosphate and carbamate compounds

• Published in: Pesticide biochemistry and physiology 2006-11, Vol.86 (3), p.162-166
• Main Authors: Zou, Ming-Qiang, Yang, Rui, Wang, Da-Ning, Li, Jin-Feng, Jin, Qin-Han
• Format: Article
• Language: English
• Subjects: Biological and medical sciences; Biotechnology; Fundamental and applied biological sciences. Psychology; Immobilization of enzymes and other molecules; Immobilization techniques; Immobilized cholinesterase; Methods and techniques. Experimental designs. Plant protection equipments; Methods. Procedures. Technologies; On-site screening; Organophosphate and carbamate compounds; Phytopathology. Animal pests. Plant and forest protection; Polyvinylpyrrolidone K30 (PVP)
• ISSN: 0048-3575; 1095-9939
• DOI: 10.1016/j.pestbp.2006.03.003

Acetylcholinesterase was physically adsorbed on polyvinylpyrrolidone K30 to prepare a novel immobilized cholinesterase, which was described for the first time. The immobilized enzyme was tested for its reactivity, enzyme activity stability, the solubility, the influence of pH and buffer, and characteristic reactivity with inhibitors, e.g., organophosphate and carbamate compounds. The result revealed that the immobilized cholinesterase held its original activity in solution, stable enough, easy to dissolve for convenient operation, and this immobilized cholinesterase could be well used for rapid, on-site screening of organophosphate and carbamate compounds, together with a portable pesticide analyzer.