Effects of fullerenol nanoparticles on acetamiprid induced cytoxicity and genotoxicity in cultured human lung fibroblasts

• Published in: Pesticide biochemistry and physiology 2014-09, Vol.114, p.1-7
• Main Authors: Çavaş, Tolga, Çinkılıç, Nilüfer, Vatan, Özgür, Yılmaz, Dilek
• Format: Article
• Language: English
• Subjects: Acetamiprid; Cell Line; Cell Survival - drug effects; Comet Assay; Cytotoxicity; Fibroblasts - drug effects; Fullerenes - pharmacology; Fullerenol; Genotoxicity; Histones - metabolism; Humans; IMR-90 cells; Insecticides - toxicity; Lung - cytology; Micronucleus Tests; Mutagens - toxicity; Nanoparticles - toxicity; Neonicotinoids; Protective Agents - pharmacology; Pyridines - toxicity
• ISSN: 0048-3575; 1095-9939
• DOI: 10.1016/j.pestbp.2014.07.008

[Display omitted] •In vitro toxicity of acetamiprid and fullerenol nanoparticles were evaluated.•Acetamiprid was both cytotoxic and genotoxic on IMR-90 cells.•Fullerenol was not cytotoxic or genotoxic on IMR-90 cells.•Co-exposure with fullerenol significantly reduced the toxicity of acetamiprid. The aim of this study was to investigate the effects of water soluble fullerene (fullerenol) nanoparticles on the in vitro genotoxicity induced by the insecticide acetamiprid. Healthy human lung cells (IMR-90) were treated with fullerenol C60(OH)n (n: 18–22) alone and in combination with acetamiprid for 24h. The micronucleus test, comet assay and γ-H2AX foci formation assays were used as genotoxicity endpoints. Cytotoxicity was evaluated using the clonogenic assay. The maximum tested concentration of fullerenol (1.600μg/ml) induced 77% survival where as the lowest concentration (25μg/ml) was not cytotoxic where as acetamiprid was cytotoxic. Fullerenol did not induce genotoxicity at tested concentrations (50–1600μg/L). On the other hand, acetamiprid (>50μM) significantly induced formation of micronuclei, and double and single stranded DNA breaks in IMR-90 cells. For simultaneous exposure studies, two non-cytotoxic concentrations (50 and 200μg/ml) of fullerenol and three cytotoxic concentrations of acetamiprid (100, 200 and 400μM) were selected. As a result, we observed that co-exposure with fullerenol significantly reduced the cytotoxicity and genotoxicity of acetamiprid in IMR-90 cells. Our results indicated the protective effect of water soluble fullerene particles on herbicide induced genotoxicity.